Composite

Part:BBa_K4623020

Designed by: Ruixiao Tan   Group: iGEM23_BNU-China   (2023-10-09)
Revision as of 13:58, 11 October 2023 by Dooooog (Talk | contribs) (→‎Usage and Biology)

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Nucleotide Silinker expression pathway(T7)

Usage and Biology

This composite part is the T7 expression pathway of Nucleotide (BBa_K4623013), which is expressed Nucleotide upon IPTG induction. For more information on Nucleotide, go to BBa_K4623013


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1099
    Illegal PstI site found at 1417
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1099
    Illegal PstI site found at 1417
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1099
    Illegal PstI site found at 1417
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1099
    Illegal PstI site found at 1417
    Illegal AgeI site found at 523
    Illegal AgeI site found at 583
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1222
    Illegal SapI.rc site found at 937


[edit]
Categories
Parameters
None