Coding

Part:BBa_K4623013:Design

Designed by: Ruixiao Tan   Group: iGEM23_BNU-China   (2023-10-09)
Revision as of 13:48, 11 October 2023 by Dooooog (Talk | contribs) (→‎Design Notes)


Nucleotide Silinker(NS)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1024
    Illegal PstI site found at 1342
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1024
    Illegal PstI site found at 1342
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1024
    Illegal PstI site found at 1342
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1024
    Illegal PstI site found at 1342
    Illegal AgeI site found at 448
    Illegal AgeI site found at 508
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1147
    Illegal SapI.rc site found at 862


Design Notes

The sequence includes the stop codon.

Source

This part sequence is primarily derived from an artificially modified amino acid sequence that has been optimized based on E. coli codons.

References