Coding
Part:BBa_K4880002:Design
Designed by: Xinran Xia Group: iGEM23_BASIS-China (2023-08-25)
AgBS
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1681
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1681
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1276
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1681
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1681
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 64
Illegal BsaI.rc site found at 505
Illegal BsaI.rc site found at 1288
Design Notes
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Source
This sequence is derived from the Abies grandis genome, and it is adapted to the cyanobacteria codon.
References
Blanc-Garin, V., Chenebault, C., Diaz-Santos, E., Vincent, M., Sassi, J.-F., Cassier-Chauvat, C., & Chauvat, F. (2022). Exploring the potential of the model cyanobacterium Synechocystis PCC 6803 for the photosynthetic production of various high-value terpenes. Biotechnology for Biofuels and Bioproducts, 15(1). https://doi.org/10.1186/s13068-022-02211-0