Translational_Unit

Part:BBa_K4879007:Design

Designed by: Rohaneshwar Manikandan   Group: iGEM23_IISER-Pune-India   (2023-10-08)
Revision as of 13:41, 11 October 2023 by Sauron 3791 (Talk | contribs)


JcFATB Expression Construct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1803
    Illegal suffix found in sequence at 2073
    Illegal EcoRI site found at 1368
    Illegal EcoRI site found at 2067
    Illegal PstI site found at 587
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1368
    Illegal EcoRI site found at 1803
    Illegal EcoRI site found at 2067
    Illegal SpeI site found at 2074
    Illegal PstI site found at 587
    Illegal PstI site found at 2088
    Illegal NotI site found at 1809
    Illegal NotI site found at 2081
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1368
    Illegal EcoRI site found at 1803
    Illegal EcoRI site found at 2067
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1803
    Illegal suffix found in sequence at 2074
    Illegal EcoRI site found at 1368
    Illegal EcoRI site found at 2067
    Illegal PstI site found at 587
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1803
    Illegal EcoRI site found at 1368
    Illegal EcoRI site found at 2067
    Illegal XbaI site found at 1818
    Illegal SpeI site found at 2074
    Illegal PstI site found at 587
    Illegal PstI site found at 2088
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The expression cassette contains overhangs on both its 5' and 3' ends that are complementary to the JcFATB and JcFATA expression cassettes respectively in order to facilitate DNA assembly with the NEBuilder HiFi DNA Assembly kit.


Source

The expression construct was synthesized by Twist Biosciences.


References