Coding
Part:BBa_K4825024
Designed by: Cai Luxi Group: iGEM23_GreatBay-SCIE (2023-10-11)
pXyl
"pXyl is a synthetic inducible promoter that is regulated by inducer xylose. It is constructed to be the minimal inducible promoter in eukaryotes.
This part is sensitive to xylose and repressed by glucose, but insensitive to other metabolites. It is associated with the xylose-dependent activator XylR. The galactose inductibility of this part has been widely used in heterologous protein expression. In our project, this part is utilized to construct a plasmid with Bax gene to fulfill the kill switch design with a logic gate induced by xylose in S.cerevisiae. "
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |