Composite

Part:BBa_K4583053

Designed by: Suiru Lu   Group: iGEM23_SDU-CHINA   (2023-10-05)
Revision as of 13:18, 11 October 2023 by Lusuiru (Talk | contribs) (→‎Usage and Biology)


PHB production Regulation: PesaS-B0034-gltA-PesaRwt-B0034-PHBcab-PYU16-B0034-SRRz

PesaS-B0034-gltA-PesaRwt-B0034-PHBcab-PYU16-B0034-SRRz

Usage and Biology

Many production processes using microorganisms face the dilemma of conflicting production products and key metabolic pathways. This means that simply introducing product-synthesising genes into an engineered bacterium can greatly affect the growth of the microorganism, leading to a situation where production is too low.

This part is about PHB Production Regulation based on an three-layer dynamic regulation model. It successfully regulate the PHB production by dividing the process into 3 phases: Growth Phase, Production Phase and Product-release Phase. In this way, the cell factory can firstly grow

1. Three-layer Dynamic Regulation Model

2. The first layer--Growth control

3. The second layer--Production control

4. The Third layer--Product-release control

Characterization

1. Protocols

2. The Characterization of PesaS and PesaRwt/PesaRc/PesaRp

3. The Characterization of PesaRwt/PesaRc/PesaRp and PYU3/PYU7/PYU16/PYU92

Summary of Results

Our part provides an example for future iGEM team

Limitations

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5826
    Illegal SapI.rc site found at 769


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Categories
Parameters
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