Coding
Part:BBa_K4825018:Design
Designed by: Qiu Tianhou Group: iGEM23_GreatBay-SCIE (2023-10-11)
pAra1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 46
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
"1. Different spacers derived from the article of synthetic promoters are placed between Inv AraI1 and AraO2 to ensure the original 193 bp interval present in natural E.coli is conserved. AraO2 is one bp upstream from TSS to explore different oppotunities. 2.Inversion of Ara operon with two AraI half sites reverses the original position of AraO. The inversion reduces the potential repressive effect due to dimeric AraC binding to AraI half sites in the Ara operon. 3.T-rich spacers results in nucleosome-free regions to reduce the potential impacts from neighbouring DNA.
"
Source
Synthesized