Coding

Part:BBa_K4825016

Designed by: Ye Siyi   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 13:14, 11 October 2023 by Registry (Talk | contribs)

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ADH7

ADH7 is derived from S.cerevisiae genome encoding for an alcohol dehydrogenase (ADH), which catalyzes the conversion of aldehydes to the corresponding alcohols. In the pathway of producing isopentanol, the termination enzymes, ketoacid decarboxylases (KDCs) and alcohol dehydrogenases (ADHs), convert n-carbon ketoacids to their corresponding (n − 1)-carbon alcohols. The part collection includes ARO10 (2-ketoacid dehydrogenase), ADH7 and ATF1 (alcohol acetyltransferase). ARO10 and ADH7 catalyze the reaction to convert 2-ketoacid to isopentanol, and ATF1 further catalyze the reaction from isopentanol to isopentyl acetate (also named isoamyl acetate). The part collection can offer an efficient pathway and inspire the future team to design the pathway of producing isoamyl acetate efficiently, and obtaining the banana-like odor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 471
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 232
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None