Coding

Part:BBa_K5004002

Designed by: Elina Wang   Group: iGEM23_BFSU-ICUnited   (2023-10-11)
Revision as of 13:10, 11 October 2023 by Registry (Talk | contribs)

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a sophisticated enzyme composed of glutathione synthetase GshF and PepG

We have embarked on the development of a sophisticated enzyme composed of glutathione synthetase GshF and PepG. GshF is proficient in catalyzing the synthesis of glutathione, safeguarding hair cells against free radical harm and shielding melanocytes from oxidative damage. On the other hand, PepG is a concise peptide with a composition of 11 polypeptides abundant in cysteine, enhancing the disulfide bonding in keratin and consequently fortifying hair elasticity and strength. To effectively segregate these two proteins, we integrated Thrombin cleavage sites between GshF and PepG. Our research began with examining the activity of GshF in isolation, followed by the construction of engineered Escherichia coli that overexpresses GSHF-PEPG. Practical tests were subsequently performed on hair specimens.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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