Coding

Part:BBa_K4623005:Design

Designed by: Ruixiao Tan   Group: iGEM23_BNU-China   (2023-10-09)
Revision as of 13:05, 11 October 2023 by Dooooog (Talk | contribs) (→‎Design Notes)


Trx-thrombin-mSA-SBP, Basical Silinker that can be purified using silica


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 427
    Illegal AgeI site found at 487
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In this part, the codon was optimized for E.coli, and the C-terminus contained a stop codon.

Source

The amino acid sequences of this part were derived from references, and most of them were artificially engineered coding sequences

References