Part:BBa_K4825008

JF m

"JFm is a mutated version of the signal peptide originated in K.smarxianu, JF, and utilized in our project's chassis, S.cerevisiae, to target heterologous proteins to the extracellular environment of yeast cells.

This part as a signal peptide can function in S.cerevisiae to enhance the secretion level of heterologous proteins or recombinant enzymes by determining and trafficing the secretion pathway of target genes,and the modifications in the genetic sequence remarkably increase the secretion level in yeasts, compared to the original JF signal peptide

In our project, this part is used to secrete heterologous protein Cry1518-35 out of S.cerevisiae, of which the efficiency is examined by secreting red fluorescence protein (RFP). This part can be applied as for a more efficient expression of heterologous proteins in S.cerevisiae than the yeast's native signal peptide, especially for the expression of large molecule proteins. "

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]