Coding

Part:BBa_K4825006:Design

Designed by: Cai Luxi   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 12:47, 11 October 2023 by Registry (Talk | contribs)

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OPT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 268
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 268
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 268
    Illegal XhoI site found at 244
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 268
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A9D and A20T mutations are made from the signal peptide ScMα to improve the production of heterologous proteins in S.cerevisiae as a universal signal peptide.


Source

Saccharomyces cerevisiae

References