Coding
Part:BBa_K4825006:Design
Designed by: Cai Luxi Group: iGEM23_GreatBay-SCIE (2023-10-11)
OPT
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 268
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 268
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 268
Illegal XhoI site found at 244 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 268
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 268
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A9D and A20T mutations are made from the signal peptide ScMα to improve the production of heterologous proteins in S.cerevisiae as a universal signal peptide.
Source
Saccharomyces cerevisiae