![](https://parts.igem.org/images/partbypart/icon_coding.png)
Part:BBa_K4825006
OPT
"OPT is an optimized version of the signal peptide originated in S.cerevisiae, ScMα, that can target heterologous proteins to the extracellular environment of yeast cells.
This part as a signal peptide can enhance the ability of S.cerevisiae in secreting heterologous or recombinant enzymes by determining and trafficing the secretion pathway of target genes, and the modifications in the genetic sequence remarkably increase the secretion level in yeasts, compared to the native ScMα signal peptide.
In our project, this part is used to secrete heterologous protein Cry1518-35 out of S.cerevisiae, of which the efficiency is examined by secreting red fluorescence protein (RFP). This part can be applied as an advanced attempt for heterologous expression in S.cerevisiae. "
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 268
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 268
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 268
Illegal XhoI site found at 244 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 268
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 268
- 1000COMPATIBLE WITH RFC[1000]
None |