Coding

Part:BBa_K4825006

Designed by: Cai Luxi   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 12:47, 11 October 2023 by Registry (Talk | contribs)

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OPT

"OPT is an optimized version of the signal peptide originated in S.cerevisiae, ScMα, that can target heterologous proteins to the extracellular environment of yeast cells.

This part as a signal peptide can enhance the ability of S.cerevisiae in secreting heterologous or recombinant enzymes by determining and trafficing the secretion pathway of target genes, and the modifications in the genetic sequence remarkably increase the secretion level in yeasts, compared to the native ScMα signal peptide.

In our project, this part is used to secrete heterologous protein Cry1518-35 out of S.cerevisiae, of which the efficiency is examined by secreting red fluorescence protein (RFP). This part can be applied as an advanced attempt for heterologous expression in S.cerevisiae. "

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 268
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 268
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 268
    Illegal XhoI site found at 244
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 268
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None