Coding

Part:BBa_K4623001

Designed by: Yixin Huang   Group: iGEM23_BNU-China   (2023-10-08)
Revision as of 12:42, 11 October 2023 by Dooooog (Talk | contribs)


Monomeric streptavidin(mSA)

Streptavidin is a tetramer protein, and one molecule of streptavidin can bind to tetramolecular biotin with high specificity. The dissociation constant of streptavidin-biotin complex is on the order of 10mol/L, which is a very perfect biotin-binding protein, and its application range is wider than that of avidin, which is also commonly used in biology. Monomeric streptavidin called mSA has been developed by scientists through amino acid mutations and has the highest affinity for biotin among current monovalent streptavidins, with an affinity of 2.8nM[1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
    Illegal AgeI site found at 142
  • 1000
    COMPATIBLE WITH RFC[1000]

References:

[1]Lim KH, Huang H, Pralle A, Park S. Stable, high-affinity streptavidin monomer for protein labeling and monovalent biotin detection. Biotechnol Bioeng. 2013 Jan;110(1):57-67. doi: 10.1002/bit.24605. Epub 2012 Aug 8. PMID: 22806584.


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