Composite

Part:BBa_K4583027

Designed by: Suiru Lu   Group: iGEM23_SDU-CHINA   (2023-10-05)
Revision as of 12:36, 11 October 2023 by Lusuiru (Talk | contribs) (→‎Characterization)


PesaS-B0034-GFP-PesaRwt-B0034-mKate-PYU3-BFP

PesaS-B0034-GFP-PesaRwt-B0034-mKate-PYU3-BFP

Usage and Biology

Characterization

Protocols

The Characterization of PesaS and PesaRwt/PesaRc/PesaRp

The Characterization of PesaRwt/PesaRc/PesaRp and PYU3/PYU7/PYU16/PYU92

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1261
    Illegal XhoI site found at 981
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2372
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 860
    Illegal BsaI.rc site found at 1926
    Illegal SapI.rc site found at 1308

Summary of Results

Limitations

[edit]
Categories
Parameters
None