Part:BBa_K4825004

Cry1518-35

"Cry1518-35 is a crystal protein gene isolated from the strain of Bacillus thuringiensis YBT-1518, performing insecticide bioactivity to nematodes. In our project, we will modify both E.coli and S.cerevisiae to express this part as an effective killing agent, in order to eliminate the parasites inside Giant African Snails, A.cantonensis.

This part has specific toxicity to more than 500 species of insects such as Lepidoptera, Diptera, Coleoptera, Hymenoptera, and Homoptera, as well as protozoa, linear animals, and flat animals. However, it is environmentally friendly and has negligible impacts on other aquatic animals, birds and mammals.

While considering the future parasitic control, Cry1518-35 can provide future iGEM teams that dedicate in achieving ecological balance with more choices of nematode pesticides. "

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 132
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]