Composite
Part:BBa_K4880018:Design
Designed by: Xinran Xia Group: iGEM23_BASIS-China (2023-09-08)
Ptrc-theo-MsLIMS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 55
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1960
Design Notes
In our design, the expression of limonene synthase is controlled by IPTG and theophylline. Transcription is regulated by IPTG and translation initiates only when there is theophylline present.
Source
This composite part combines BBa_K3930029 and BBa_J435283.