Coding

Part:BBa_K4698008

Designed by: Junjie Zhu   Group: iGEM23_HS-Shanghai   (2023-10-11)
Revision as of 11:30, 11 October 2023 by Registry (Talk | contribs)

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T7 RNA polymerase has a protein degradation tag attached to its C-terminus

T7 RNA polymerase has a protein degradation tag (degron) attached to its C-terminus, which allows rapid degradation of T7 RNA polymerase, so the T7 promoter cannot be transcribed. Its commonly used in Phage-assisted continuous (PACE).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None