Composite

Part:BBa_K4800050

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-08)
Revision as of 11:25, 11 October 2023 by ZQM 0907 (Talk | contribs)

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pTrc99a-YahK-SAV2585

We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene SAV2585 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-SAV2585.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 4316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 4316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3624
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 4316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 4316
    Illegal NgoMIV site found at 3852
    Illegal NgoMIV site found at 4516
    Illegal NgoMIV site found at 4545
    Illegal NgoMIV site found at 4590
    Illegal NgoMIV site found at 5394
    Illegal NgoMIV site found at 5508
    Illegal AgeI site found at 4201
    Illegal AgeI site found at 4252
    Illegal AgeI site found at 4327
    Illegal AgeI site found at 4441
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5781
    Illegal BsaI site found at 6849
    Illegal SapI.rc site found at 767


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