Composite

Part:BBa_K4800049

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-08)
Revision as of 11:19, 11 October 2023 by ZQM 0907 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pTrc99a-YahK-Spo3471

We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene Spo3471 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-Spo3471.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3808
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3808
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3624
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3808
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3808
    Illegal NgoMIV site found at 4468
    Illegal AgeI site found at 4455
    Illegal AgeI site found at 4579
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5346
    Illegal BsaI site found at 6414
    Illegal SapI.rc site found at 767


[edit]
Categories
Parameters
None