Regulatory

Part:BBa_K4907020

Designed by: Linhao Zhang   Group: iGEM23_XMU-China   (2023-09-13)
Revision as of 10:38, 11 October 2023 by Haoxihuan (Talk | contribs)

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araBAD promoter

Biology

The araBAD promoter is an E. coli promoter that is tightly controlled by inducer: L-arabinose.

Usage and design

We used This part to combine hrpR(BBa_K4907021) and hrpS(BBa_K4907022) to construct the hrp system and obtained the composite part BBa_K4907126, which are assembled on the expression vector pSB1C3(Fig. 1).

Fig. 1 Gene circuits of the composite part BBa_K4907126

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 300
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56


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Categories
Parameters
None