Coding

Part:BBa_K4808002

Designed by: Zhao Guichun   Group: iGEM23_AIS-China   (2023-10-11)
Revision as of 10:22, 11 October 2023 by Registry (Talk | contribs)

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ilvA*

For further improvement on a-kb production levels, we want to optimize threonine's conversion into a-kb, a key step in a-kb production. For this goal, we tried using another plasmid of a different copy number to express ilvA. We also site mutated ilvA (obtainning ilvA*) to make its expressed enzymes resistant to the inhibition of Isoleucine. Isoleucine is a downstream product of a-kb. Our gene knock out choices do not completely obstruct the pathway continuing downwards of a-kb, thus meaning that Isoleucine is existent in the E.coli cell (Isoleucine inhibits ilvA expressed Threonine Dehydrase, thus negatively affecting Threonine's conversion into a-kb, which is reported in many papers).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1315
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1315
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1315
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1315
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1315
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1168


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Parameters
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