Part:BBa_K4960038

pPVC promoter->pvc1-pvc12->pvc13_NTD-1*EAAAK-CKGGRAKDC-1*EAAAK -pVc13_CTD->pvc14-pvc16

Properties

A flexible linker which can influence the structure of tail fiber connected to the CKGGRAKDC.

Usage and Biology

The system is a syringe-like macromolecular complex that inject protein payloads into fat cells by driving a spike through the cellular membrane. Different payloads can be load into the lumen of the inner tube behind the spike, form fusion proteins with the tube, or associate with the spike itself. Studies suggest that the tail fibre is probably involved in target recognition. [1]Our project has enabled the system to target adipocytes by modifying the tail fibre to bind to CKGGRAKDC. CKGGRAKDC is a peptide motif that homes to white fat vasculature. The studies show that CKGGRAKDC targets the white adipose vasculature without a detectable preference for any particular anatomical white fat depot. Meanwhile, the motif is preferentially internalized by a receptor in the adipose vasculature that may serve for targeted delivery of therapeutic compounds to fat.[2] After specific recognition and binding to adipocytes, it can cross the via sheath contraction and inject the desired payloads. The linker 1*EAAAK on both sides of CKGGRAKDC can affect the function and efficiency of bond and recognition by affecting the structure of the tail protein.

Figure1: Schematic diagram of the influence of different Linkers on the structure of tail fibers
Figure2: PVC illustration

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7406
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2407
  Illegal BglII site found at 11264
  Illegal BglII site found at 14099
  Illegal BglII site found at 16960
  Illegal XhoI site found at 2691
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 11792
  Illegal NgoMIV site found at 17491
  Illegal NgoMIV site found at 17630
  Illegal AgeI site found at 6704
  Illegal AgeI site found at 9641
  Illegal AgeI site found at 10327
  Illegal AgeI site found at 10468
  Illegal AgeI site found at 11576
  Illegal AgeI site found at 17929
  Illegal AgeI site found at 19617
  
• 1000
  COMPATIBLE WITH RFC[1000]