Composite

Part:BBa_K4790105

Designed by: Yutong Ji   Group: iGEM23_NNU-CHINA   (2023-10-11)
Revision as of 10:09, 11 October 2023 by Registry (Talk | contribs)

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ASR1(node 59)-W120R/pET-22b(+)

The DNA fragment has been site-directed mutated through PCR. The 120th amino acid in expression product of node 59 was mutated from tyrosine to arginine.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 960
    Illegal PstI site found at 261
    Illegal PstI site found at 393
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 960
    Illegal NheI site found at 882
    Illegal NheI site found at 1113
    Illegal PstI site found at 261
    Illegal PstI site found at 393
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 960
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 960
    Illegal PstI site found at 261
    Illegal PstI site found at 393
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 960
    Illegal PstI site found at 261
    Illegal PstI site found at 393
    Illegal NgoMIV site found at 143
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None