Composite

Part:BBa_K4613311

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)
Revision as of 09:55, 11 October 2023 by Jiandong (Talk | contribs)


pET-PC-SUMO-M-CPA

The composite part was constructed to achieve solube expression of MCPA and analyze the function of Mature Carboxypeptidase A (M-CPA). The composite part can be directly imported into plasmid and express M-CPA at the same time.

Reference

  1. Xiong L, Peng M, Zhao M, et al. Truncated expression of a carboxypeptidase a from bovine improves its enzymatic properties and detoxification efficiency of ochratoxin A[J]. Toxins, 2020, 12(11): 680.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 152
    Illegal BglII site found at 474
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 584
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
chassis
function