Part:BBa_K4613303

pET-29a(+)-T3-ADH3

In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried the T7 lac promoter from pET-29a (+). The composite part can be directly imported into plasmid and express T3-ADH3 induced with IPTG.

Reference

1. Dai Z, Yang X, Wu F, et al.Living fabrication of functional semi-interpenetrating polymeric materials[J].Nat Commun,2021, 12 (1): 3422.
2. Zakeri B, Fierer J O, Celik E, et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proc Natl Acad Sci U S A,2012, 109 (12): E690-7.
3. Reddington S C, Howarth M.Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher[J].Curr Opin Chem Biol,2015, 29: 94-9.
4. Dai L, Niu D, Huang J W, et al.Cryo-EM structure and rational engineering of a superefficient ochratoxin A-detoxifying amidohydrolase[J].J Hazard Mater,2023, 458: 131836.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1007
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1742
  Illegal AgeI site found at 1430
  Illegal AgeI site found at 1592
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 75