Composite

Part:BBa_K4613303

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)
Revision as of 09:35, 11 October 2023 by Jiandong (Talk | contribs) (→‎Reference)


pET-29a(+)-T3-ADH3

In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried the T7 lac promoter from pET-29a (+). The composite part can be directly imported into plasmid and express T3-ADH3 induced with IPTG.

Reference

  1. Reddington S C, Howarth M.Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher[J].Curr Opin Chem Biol,2015, 29: 94-9.
  2. Xiong L, Peng M, Zhao M, et al.Truncated Expression of a Carboxypeptidase A from Bovine Improves Its Enzymatic Properties and Detoxification Efficiency of Ochratoxin A[J].Toxins (Basel),2020, 12 (11).
  3. Dai Z, Yang X, Wu F, et al.Living fabrication of functional semi-interpenetrating polymeric materials[J].Nat Commun,2021, 12 (1): 3422.

Zakeri B, Fierer J O, Celik E, et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proc Natl Acad Sci U S A,2012, 109 (12): E690-7. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1007
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1742
    Illegal AgeI site found at 1430
    Illegal AgeI site found at 1592
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 75


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