Composite

Part:BBa_K4800043

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-08)
Revision as of 08:57, 11 October 2023 by ZQM 0907 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pTrc99a-SAV2585

We linearized the pTrc99a vector using restriction endonuclease and connected the target gene SAV2585 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-SAV2585.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4486
    Illegal PstI site found at 3174
    Illegal PstI site found at 4498
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3174
    Illegal PstI site found at 4498
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4480
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4486
    Illegal PstI site found at 3174
    Illegal PstI site found at 4498
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4486
    Illegal PstI site found at 3174
    Illegal PstI site found at 4498
    Illegal NgoMIV site found at 2710
    Illegal NgoMIV site found at 3374
    Illegal NgoMIV site found at 3403
    Illegal NgoMIV site found at 3448
    Illegal NgoMIV site found at 4252
    Illegal NgoMIV site found at 4366
    Illegal AgeI site found at 3059
    Illegal AgeI site found at 3110
    Illegal AgeI site found at 3185
    Illegal AgeI site found at 3299
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4682
    Illegal BsaI site found at 5750
    Illegal SapI.rc site found at 767


[edit]
Categories
Parameters
None