Composite

Part:BBa_K4800041

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-08)
Revision as of 08:41, 11 October 2023 by ZQM 0907 (Talk | contribs)

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pTrc99a-PatA

We linearized the pTrc99a vector using restriction endonuclease and connected the target gene PatA to it through homologous recombination to obtain the recombinant plasmid pTrc99a-PatA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4036
    Illegal PstI site found at 4048
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 4048
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4030
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4036
    Illegal PstI site found at 4048
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4036
    Illegal PstI site found at 4048
    Illegal NgoMIV site found at 2804
    Illegal AgeI site found at 3311
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4232
    Illegal BsaI site found at 5300
    Illegal SapI.rc site found at 767


[edit]
Categories
Parameters
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