Part:BBa_K4767018

Pci-RBS-ptpA-TT

Uses a promoter PcI(BBa_R0051) ,strong RBS(BBa_J34801), ptpA(BBa_K4767002)and TT(BBa_B0015). This part is an easy BioBrick.

Usage and Biology

This part is used to express ptpA gene in the mutant strain as a complementation to verify its function in the Shewanella oneidensis.

We created the ptpA knockout mutant and complementation strain to verify its function in the formation and conductivity of S. oneidensis MR-1 biofilm. Our results show that the ability of the mutant strain to form biofilm on both conductive (well plate) and non-conductive (anode) surfaces significantly reduced, whereas the complementation strain restored this capability. In microbial fuel cells(MFC), maximum voltage of the mutant strain raised about 36% compared to the wild type with the lower internal resistance In summary, we come to the conclusion that ptpA gene in S. oneidensis MR-1 may possibly effect the biofilm formation and it’s conductivity by meditating the polysaccharide biosynthesis.

The growth curves of the wild-type(WT), ∆ptpA and the complementaion strain(Fig A) show that ptpA gene does not effect the cell growth. The biofilm formation of three strains in non-conductive 24-well plates(Fig B) and MFC anodes(Fig D). The ability for the mutant strain to form biofilm is reduced compared to the WT and complementaion in both surfaces. With less biofilm in the electrode, the mutant strain can create a higher current in MFC(Fig C), and reduce the MFC internal resistance(Fig E).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]