Composite

Part:BBa_K4790086

Designed by: Hongyan Jing   Group: iGEM23_NNU-CHINA   (2023-10-11)
Revision as of 08:21, 11 October 2023 by Registry (Talk | contribs)

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ASR6(node 112)/pET-22b(+)

ASR5 is constructed based on pET-22b. We amplified pET-22b by PCR and use Gibson Assembly method. DNA fragment node 112 was subcloned into pET-22b vector between T7 promoter and T7 terminator. The antibiotic marker of pET-22b is ampicillin resistance gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 442
    Illegal PstI site found at 453
    Illegal PstI site found at 579
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 429
    Illegal NheI site found at 960
    Illegal PstI site found at 442
    Illegal PstI site found at 453
    Illegal PstI site found at 579
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 449
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 442
    Illegal PstI site found at 453
    Illegal PstI site found at 579
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 442
    Illegal PstI site found at 453
    Illegal PstI site found at 579
    Illegal NgoMIV site found at 143
    Illegal NgoMIV site found at 425
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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