Composite

Part:BBa_K283006:Design

Designed by: Nelson Chu, W Ling , Shi Lei   Group: iGEM09_HKU-HKBU   (2009-10-13)
Revision as of 01:32, 20 October 2009 by NelsonChu (Talk | contribs) (→‎Design Notes)

cheZ-his tag-double terminators


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 646
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The chez coding region contain a 6-histidine tag

Source

The CI, terminator, pLambda promoter and the chez gene are solf designed the pLux promoter, rbs are from the registry

References