Composite

Part:BBa_K4604027

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-10)
Revision as of 07:31, 11 October 2023 by HannahSwientek (Talk | contribs)

piG_K12BSb (LacIprom_riboK12_T7RBS_lacI_trcProm_sfGFP)

piG_K12BSb consists of the LacI promoter, the AdoCbl riboswitch from E. coli K12, the modified T7 RBS, lacI and the trc promoter. The trc promoter can be induced with IPTG. This BioBrick is a sensor for AdoCbl (a bioavailable form of B12) with a variable marker gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 215
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None