Coding

Part:BBa_K4630006:Design

Designed by: Shuangwu Wu   Group: iGEM23_WHU-China   (2023-10-10)
Revision as of 07:25, 11 October 2023 by Shuangwu (Talk | contribs) (→‎References)


Cas9 in pCas plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1344
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1344
    Illegal NheI site found at 1103
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1344
    Illegal BamHI site found at 3382
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1344
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1344
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because the Cas9 sequence in origin part is not consistent with our plasmid, so we found this part.


Source

Zhao, D., Yuan, S., Xiong, B. et al. Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9. Microb Cell Fact 15, 205 (2016).

References

Zhao, D., Yuan, S., Xiong, B. et al. Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9. Microb Cell Fact 15, 205 (2016)