![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4800055
Designed by: Yifei Wu Group: iGEM23_NJTech-China-B (2023-10-11)
pRSFDuet-sfp-MmCAR-YahK
We used pACYCDuet-sfp-MmCAR-YahK as a template and amplified the PT7-YciA-Ptrc-sfp-MmCAR-Yahk fragment by PCR, and homologous recombination of PT7-YciA-Ptrc-sfp-MmCAR-Yahk with the linearized pRSFDuet vector was carried out by the In-fusion cloning method to yield plasmid of pRSFDuet-sfp-MmCAR-YahK.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4145
Illegal XbaI site found at 1415
Illegal PstI site found at 4580
Illegal PstI site found at 4607
Illegal PstI site found at 5876
Illegal PstI site found at 5903
Illegal PstI site found at 6296
Illegal PstI site found at 6792
Illegal PstI site found at 7436 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4145
Illegal XbaI site found at 1415
Illegal PstI site found at 4580
Illegal PstI site found at 4607
Illegal PstI site found at 5876
Illegal PstI site found at 5903
Illegal PstI site found at 6296
Illegal PstI site found at 6792
Illegal PstI site found at 7436
Illegal NgoMIV site found at 6404
Illegal NgoMIV site found at 6466
Illegal NgoMIV site found at 6683
Illegal NgoMIV site found at 7130
Illegal AgeI site found at 4310
Illegal AgeI site found at 5321
Illegal AgeI site found at 5618
Illegal AgeI site found at 6503
Illegal AgeI site found at 6512
Illegal AgeI site found at 7187
Illegal AgeI site found at 9049 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 9122
Illegal SapI site found at 660
[edit]
Categories
Parameters
None |