Part:BBa_K4785005:Experience

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Applications of BBa_K4785005

1.Protein function verification

In the inhibition of biofilm growth experiment (Fig.1), we can observe that PslG(BBa_K4785004) treatment group effectively inhibited the growth of biofilm by comparing to the control group. HMGB1-A box shows the strongest ability to inhibit the biofilm growth among four HMGB1 modified proteins.

In the biofilm disassembly experiment (Fig.2), the PslG treatment group reduced the OD550 absorption value corresponding to the biofilm biomass from about 1.5 to about 0.5 in the first two hours, while HMGB1-A box shows the strongest ability to disassemble the biofilm among four HMGB1 modified proteins.

According to those results, we decided to choose PslG and HMGB1-A box as our functional proteins.

User Reviews

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