Composite

Part:BBa_K4800053

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-09)
Revision as of 06:40, 11 October 2023 by Sariel (Talk | contribs)

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pACYCDuet-sfp-MmCAR-YahK

We used codon-optimized and synthesized to obtain the fragment of sfp-MmCAR, which was then ligated into the pACYCDuet plasmid under the control of trc promoter. The Ptrc-YahK fragment was amplified with plasmid pTrc99a-YahK as the template, and In-Fusion cloning was then used to ligate into the above vectors to yield plasmid pACYCDuet-sfp-MmCAR-YahK.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4801
    Illegal XbaI site found at 2084
    Illegal PstI site found at 5236
    Illegal PstI site found at 5263
    Illegal PstI site found at 6532
    Illegal PstI site found at 6559
    Illegal PstI site found at 6952
    Illegal PstI site found at 7448
    Illegal PstI site found at 8092
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4801
    Illegal NheI site found at 1243
    Illegal NheI site found at 8360
    Illegal PstI site found at 5236
    Illegal PstI site found at 5263
    Illegal PstI site found at 6532
    Illegal PstI site found at 6559
    Illegal PstI site found at 6952
    Illegal PstI site found at 7448
    Illegal PstI site found at 8092
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4801
    Illegal BglII site found at 5790
    Illegal BglII site found at 6416
    Illegal BglII site found at 6692
    Illegal BglII site found at 7383
    Illegal BamHI site found at 4773
    Illegal XhoI site found at 8332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4801
    Illegal XbaI site found at 2084
    Illegal PstI site found at 5236
    Illegal PstI site found at 5263
    Illegal PstI site found at 6532
    Illegal PstI site found at 6559
    Illegal PstI site found at 6952
    Illegal PstI site found at 7448
    Illegal PstI site found at 8092
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4801
    Illegal XbaI site found at 2084
    Illegal PstI site found at 5236
    Illegal PstI site found at 5263
    Illegal PstI site found at 6532
    Illegal PstI site found at 6559
    Illegal PstI site found at 6952
    Illegal PstI site found at 7448
    Illegal PstI site found at 8092
    Illegal NgoMIV site found at 7060
    Illegal NgoMIV site found at 7122
    Illegal NgoMIV site found at 7339
    Illegal NgoMIV site found at 7786
    Illegal AgeI site found at 57
    Illegal AgeI site found at 1329
    Illegal AgeI site found at 1652
    Illegal AgeI site found at 4966
    Illegal AgeI site found at 5977
    Illegal AgeI site found at 6274
    Illegal AgeI site found at 7159
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
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Parameters
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