Composite

Part:BBa_K4800035

Designed by: Yao Yao   Group: iGEM23_NJTech-China-B   (2023-10-08)
Revision as of 06:39, 11 October 2023 by ZQM 0907 (Talk | contribs)

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pRSFDuet-MSMEG5739

We linearized the pRSFDuet vector using restriction endonuclease and connected the target gene MSMEG5739 to it through homologous recombination to obtain the recombinant plasmid pRSFDuet-MSMEG5739.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1415
    Illegal XbaI site found at 7230
    Illegal PstI site found at 7242
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 7242
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4705
    Illegal BamHI site found at 3574
    Illegal BamHI site found at 7224
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1415
    Illegal XbaI site found at 7230
    Illegal PstI site found at 7242
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1415
    Illegal XbaI site found at 7230
    Illegal PstI site found at 7242
    Illegal NgoMIV site found at 3823
    Illegal NgoMIV site found at 4418
    Illegal NgoMIV site found at 4426
    Illegal NgoMIV site found at 4838
    Illegal NgoMIV site found at 6938
    Illegal AgeI site found at 4565
    Illegal AgeI site found at 4883
    Illegal AgeI site found at 6121
    Illegal AgeI site found at 7653
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7474
    Illegal BsaI site found at 7726
    Illegal SapI site found at 660


[edit]
Categories
Parameters
None