Plasmid_Backbone

Part:BBa_K4765026

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-08)
Revision as of 06:06, 11 October 2023 by Siliang Zhan (Talk | contribs) (→‎Usage and Biology)


OpenV3

contributed by Fudan iGEM 2023

Usage and Biology

Robust, flexible E. coli cloning vector. Designed to be compatible with multiple cloning strategies including restriction ligation, Gibson assembly, SLiCE, and Golden Gate assembly using the FreeGenes MoClo standard. See details in stanford.freegenes.org.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2089


[edit]
Categories
Parameters
None