Part:BBa_K4879002
CvFAP
The fatty acid photodecarboxylase from Chlorella variabilis. [1]
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Usage and Biology
The fatty acid photodecarboxylase, found mainly in the chloroplasts of microalga Chlorella variabilis, catalyses the decarboxylation of free fatty acids into n-alkanes or n-alkenes in the presence of blue light. The reaction is initiated by electron abstraction from the fatty acid to a photo-excited cofactor flavin adenine dinucleotide (FAD).
The direct conversion of free fatty acids (FFAs) into n-alkanes and n-alkenes is an essential feature of our project as it bypasses a series of more expensive and intensive steps in fuel (hydrocarbon) production.
Design
The transcriptional unit for CvFAP (BBa_K4879008) is designed with the coding sequence flanked by the TEF1 promoter (BBa_K3629001) upstream and the XPR2 terminator (BBa_K3629004) downstream.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 259
Illegal PstI site found at 691
Illegal PstI site found at 958 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 259
Illegal PstI site found at 691
Illegal PstI site found at 958 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1424
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 259
Illegal PstI site found at 691
Illegal PstI site found at 958 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 259
Illegal PstI site found at 691
Illegal PstI site found at 958
Illegal NgoMIV site found at 1579
Illegal AgeI site found at 1555 - 1000COMPATIBLE WITH RFC[1000]
References
1. Bruder, S., Moldenhauer, E.J., Lemke, R.D. et al. Drop-in biofuel production using fatty acid photodecarboxylase from Chlorella variabilis in the oleaginous yeast Yarrowia lipolytica. Biotechnol Biofuels 12, 202 (2019). https://doi.org/10.1186/s13068-019-1542-4
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