DNA

Part:BBa_K4800010

Designed by: Wenbin Zhao   Group: iGEM23_NJTech-China-B   (2023-10-06)
Revision as of 05:25, 11 October 2023 by Sariel (Talk | contribs)

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ackA-pta Donor

AckA-pta Donor is exogenous DNA used by cells as a template for homologous recombination repair (HDR) after SgRNA and Cas9 complexes have caused DNA double-strand breaks at the target site.


Document

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Fig1.ackA-pta Donor

The genes at both end of the ackA-pta gene were selected as the upper and downer homology arms, which were amplified separately, purified and recovered, then joined by overlap to obtain the ackA-pta Donor.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 969
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 969
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 969
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 969
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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