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Coding
Part:BBa_K5013003
Designed by: Zhang Yuxi Group: iGEM23_Squirrel-CHN (2023-10-11)
This is a phenylalanine degrading enzyme controlled by a hypoxic promoter
Considering the functionality of phenylalanine lyase (PAL) in an anaerobic environment, we decided to position PAL downstream of the hypoxia promoter (pPepT), as this part allows for the regulation of PAL expression in such conditions. Ultimately, this composite part facilitates the transformation of phenylalanine into trans-cinnamic acid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2346
Illegal BamHI site found at 608
Illegal XhoI site found at 659
Illegal XhoI site found at 722
Illegal XhoI site found at 740
Illegal XhoI site found at 818
Illegal XhoI site found at 1019
Illegal XhoI site found at 1262
Illegal XhoI site found at 2009 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1178
Illegal NgoMIV site found at 1424
Illegal NgoMIV site found at 1592
Illegal NgoMIV site found at 1735
Illegal NgoMIV site found at 2069 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 719
Illegal BsaI site found at 1049
Illegal BsaI site found at 1055
Illegal BsaI.rc site found at 2180
Illegal SapI site found at 1009
[edit]
Categories
Parameters
None |