Coding

Part:BBa_K5013003

Designed by: Zhang Yuxi   Group: iGEM23_Squirrel-CHN   (2023-10-11)
Revision as of 05:21, 11 October 2023 by Registry (Talk | contribs)

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This is a phenylalanine degrading enzyme controlled by a hypoxic promoter

Considering the functionality of phenylalanine lyase (PAL) in an anaerobic environment, we decided to position PAL downstream of the hypoxia promoter (pPepT), as this part allows for the regulation of PAL expression in such conditions. Ultimately, this composite part facilitates the transformation of phenylalanine into trans-cinnamic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2346
    Illegal BamHI site found at 608
    Illegal XhoI site found at 659
    Illegal XhoI site found at 722
    Illegal XhoI site found at 740
    Illegal XhoI site found at 818
    Illegal XhoI site found at 1019
    Illegal XhoI site found at 1262
    Illegal XhoI site found at 2009
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1178
    Illegal NgoMIV site found at 1424
    Illegal NgoMIV site found at 1592
    Illegal NgoMIV site found at 1735
    Illegal NgoMIV site found at 2069
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 719
    Illegal BsaI site found at 1049
    Illegal BsaI site found at 1055
    Illegal BsaI.rc site found at 2180
    Illegal SapI site found at 1009


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