Coding

Part:BBa_K4800004

Designed by: Yao Yao   Group: iGEM23_NJTech-China-B   (2023-10-06)
Revision as of 05:09, 11 October 2023 by Sariel (Talk | contribs)

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MmCAR(Q302E)

MmCAR is a carboxylate reductase. In order to improve the catalytic activity of 5-hydroxyvaleric acid, we decided to mutate the 302nd amino acid in MmCAR to convert glutamine (Q) into glutamic acid (E). By changing the population of the residuals, we evaluated the effect of the mutations on MmCAR catalytic activity.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 427
    Illegal PstI site found at 454
    Illegal PstI site found at 1723
    Illegal PstI site found at 1750
    Illegal PstI site found at 2143
    Illegal PstI site found at 2639
    Illegal PstI site found at 3283
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 427
    Illegal PstI site found at 454
    Illegal PstI site found at 1723
    Illegal PstI site found at 1750
    Illegal PstI site found at 2143
    Illegal PstI site found at 2639
    Illegal PstI site found at 3283
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 981
    Illegal BglII site found at 1607
    Illegal BglII site found at 1883
    Illegal BglII site found at 2574
    Illegal XhoI site found at 3523
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


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