Coding

Part:BBa_K5013001

Designed by: Zhang Yuxi   Group: iGEM23_Squirrel-CHN   (2023-10-11)
Revision as of 05:02, 11 October 2023 by Registry (Talk | contribs)

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TP901 recognizes

This sequence contains the TP901 recognition sites attB (5' terminal) and attP (3' terminal). Where the Target sequence is replaced with the reverse T7 promoter. When TP901 recognizes this sequence, it turns over the T7 promoter to initiate downstream gene expression. In order to avoid mutual interference between the 3' terminal region of the promoter and the 5' terminal of the ORF, we added an insulator RiboJ downstream of the attP recognition site.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 92
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 92
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 92
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 73


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Categories
Parameters
None