Coding

Part:BBa_M45424:Experience

Designed by: Richard Klein   Group: UtahState_BE5930_-_S14   (2018-04-25)
Revision as of 03:36, 11 October 2023 by Wuqingyu (Talk | contribs)

Contribution of OUC-China

Group: iGEM Team OUC-China 2023

Author: Qingyu Wu

Summary: Exploring the expression conditions of the protein 

In order to explore the induction conditions of this protein, we set different concentrations of IPTG and analyzed the SDS-PAGE results using software. Finally, we obtained the following table, which shows that there is not much difference in protein expression levels at three different concentrations, but the optimal concentration is 0.5mM.

Fig 1 Expression intensity of proteins at different induction concentrations




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