DNA

Part:BBa_K5008000

Designed by: Chengyao Yin   Group: iGEM23_Squirrel-Shanghai   (2023-10-10)
Revision as of 02:12, 11 October 2023 by Registry (Talk | contribs)

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CsgA_MBP

The component BBa_K5008000 adds an additional DNA sequence based on BBa_K4161010. This DNA sequence will interact with BBa_K4161010 and give BBa_K4161010 new functions. Specifically, this part is fused with a metal binding protein (MBP) based on CsgA. At the same time, CsgA is a very important subunit of the E. coli peritrichous flagellum and participates in the assembly of the flagellum. Therefore, our part can enable the E. coli peritrichous flagellum to present MBP and play a metal-binding function.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 371
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 371
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 371
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 371
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None