Composite

Part:BBa_K4863003

Designed by: Zhihan Emma Chen   Group: iGEM23_Keystone   (2023-10-10)
Revision as of 01:37, 11 October 2023 by EmmaChen (Talk | contribs)


PpsbA2_SpyTag_Slp

A SpyTag-Slp protein complex driven by a light-inducible promoter. SpyTag (BBa_K1159200) binds to the N-terminus of surface layer protein (Slp:BBa_K4863002) of Synechocystis PCC 6803 to be displayed on the cell surface of the engineered organism.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3552
    Illegal EcoRI site found at 5532
    Illegal XbaI site found at 758
    Illegal PstI site found at 874
    Illegal PstI site found at 4474
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3552
    Illegal EcoRI site found at 5532
    Illegal NheI site found at 1181
    Illegal NheI site found at 1278
    Illegal PstI site found at 874
    Illegal PstI site found at 4474
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3552
    Illegal EcoRI site found at 5532
    Illegal BglII site found at 2693
    Illegal BamHI site found at 3599
    Illegal BamHI site found at 4913
    Illegal XhoI site found at 816
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3552
    Illegal EcoRI site found at 5532
    Illegal XbaI site found at 758
    Illegal PstI site found at 874
    Illegal PstI site found at 4474
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3552
    Illegal EcoRI site found at 5532
    Illegal XbaI site found at 758
    Illegal PstI site found at 874
    Illegal PstI site found at 4474
    Illegal AgeI site found at 2580
    Illegal AgeI site found at 2763
    Illegal AgeI site found at 3111
    Illegal AgeI site found at 3171
    Illegal AgeI site found at 4692
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None