Composite

Part:BBa_K4604041

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-10)
Revision as of 00:16, 11 October 2023 by Registry (Talk | contribs)

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piG_11 (tetR_kanR_riboK12_mazF)

Construct piG_11 is made up of the tet-promoter (consisting of the tet repressor and tet operator), that can be induced with doxycycline, the modified T7 RBS, a kanamycine resistance, a riboswitch for AdoCbl, mazF (toxin) and the rrnB terminator. The backbone we used in our experiments was pGGAselect. The kanamycine resistance was taken from iGEM UCDavis 2021 (), the tet operator from iGEM Freiburg 2022 (BBa_K4229059).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 913
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 913
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 913
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 913
    Illegal NgoMIV site found at 1364
    Illegal NgoMIV site found at 1700
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2335
    Illegal SapI.rc site found at 1213
    Illegal SapI.rc site found at 1423


[edit]
Categories
Parameters
None