Protein_Domain

Part:BBa_K4895052

Designed by: Isabella Lirtzman   Group: iGEM23_ASU   (2023-10-09)
Revision as of 00:12, 11 October 2023 by Phjiang (Talk | contribs) (→‎DeHa2 (Delftia Acidovorans))

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DeHa2 (Delftia Acidovorans)

Haloacid Dehalogenase originating from Delftia Acidovorans, belonging to the α/β hydrolase superfamily. Likely candidate for performing PFAS degradation due to Delftia acidorovans’s ability to subsist solely off of PFOA as a carbon source (Harris, et. al 2022)(10.1016/j.synbio.2022.02.005).

The sequence was optimized for the organism E.coli (K12). The GC level was optimized to be Medium (0.33 to 0.66). Existing cutsites were removed and no new ones were created for the following enzymes: BbsI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None