Part:BBa_K4895000

Salmonella Enterica LT2 strain fadA (3-ketoacyl-CoA thiolase)

This sequence encodes a specific enzyme from the Salmonella Beta-Oxidation cycle. fadB is one of many enzymes under the fad family, which largely represents enzymes associated with fatty acid metabolism. In the Beta Oxidation cycles of Salmonella and E.coli, fadB acts as the large subunit in the tetramer fadBA, or tri-functional enzyme complex fadBA. The tetramer consists of two large subunits (fadB) and two small subunits (fadA). The fadBA complex is a part of the aerobic respiration cycle in Salmonella. It is shown in literature to be less sterically specific compared to that of E.coli (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1347308/)

The Beta-Oxidation in E.coli can be broken down into four main parts:

• 1)acyl-CoA dehydrogenase (ACD)
• 2) 2-enoyl-CoA hydratase (ECH)
• 3)3-hydroxyacyl-CoA dehydrogenase (HACD)
• 4)3-ketoacyl-CoA thiolase (KACT)


fadBA is responsible for the latter three reactions, and fadA is responsible for the 3-ketoacyl-CoA thiolase. The activity of this enzyme can be explored here: (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329746/)


This part should always be used in conjunction with the fadA gene of Salmonella Enterica as well as the fadE gene of Salmonella Enterica. These three genes represent the complete beta-oxidation cycle of Salmonella Enterica. It should be used with the specificity of beta-oxidation in mind, and it only acts upon acylated fatty acids. It functions by removing two carbons at a time, and then the end product is transferred to a third enzyme, fadE. fadE recouples the biproducts of the breakdown of fatty acids, and either continues to break down the fatty acid into acetyl-coA, or feeds the long-chain fatty acid into the membrane repair cycle.


It is important to note that fadB in particular is also associated with unsaturated fatty acid synthesis, as a part of Salmonella Enterica's fab cycle.

Sequence and Features




Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]